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KMID : 0545119970070030197
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Journal of Microbiology and Biotechnology
1997 Volume.7 No. 3 p.197 ~ p.199
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Production of Indigo and Indirubin by Escherichia coli Containing a Phenol Hydroxylase Gene of Bacillus stearothermophilus
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KIM, IN-CHEOL
CHANG, HAE-CHOON/ORIEL, PATRICK
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Abstract
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Escherichia coli recombinants containing the cloned phenol hydroxylase gene of Bacillus stearothermophilus BR219 were shown to produce both indigo and its structural isomer indirubin during culture on LB broth. The ratio of indirubin/indigo was highest under conditions of prolonged culture and reduced culture oxygenation.
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